And what if I use bitcoin-QT wallet? Would a paper backup of such wallet become invalid instantly, or only after 99 more transactions?
You paper wallet contains (or may contain) one private key, corresponding to address A. If you send the funds at that address to another address B, and the change is sent to address C, then the paper wallet is rendered useless. If you send the funds at that address to another address B, and the change is sent to address A, then you keep the rest of the funds.