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Author Topic: “Cryptocurrency system using body activity data” pat. n. = WO2020060606A1  (Read 563 times)
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February 14, 2026, 04:58:54 PM
Last edit: February 14, 2026, 05:32:54 PM by ESG
 #21


   This paper reviews how synthetic biology is being used to create next-generation biosensors
 for healthcare and environmental monitoring—systems built from engineered DNA, RNA,
 and proteins that can sense diseases or chemicals and then generate easy-to-read
 signals like fluorescence, color changes, or electrical outputs.

  It highlights CRISPR-based diagnostics for rapidly detecting genetic material such as
 antibiotic-resistant bacteria and SARS-CoV-2 in COVID testing (self applied microneedle
 DNA monitoring system), biosensors that embed freeze-dried cell-free reactions
 into flexible skin-mounted devices for continuous health tracking, and even in-body or
 semi-implantable sensors designed to monitor circulating DNA or drug levels inside patients.
The review also discusses signal-boosting strategies using reporter proteins— including
 luciferase-based designs—alongside RNA switches and genetic circuits to make detection
 faster and more sensitive. Overall, the authors present these technologies as a path toward  
 quicker, personalized, and portable diagnostics, while stressing that safety, reliability,
 and large-scale deployment remain major challenges before routine clinical use.


 Provided below is a section-by-section overview of the paper:

"Synthetic biology-driven biosensors for healthcare applications: A roadmap toward programmable and intelligent diagnostics"

https://www.sciencedirect.com/science/article/abs/pii/S0956566325009121

What is Synthetic Biology⁉️

Synthetic biology is an interdisciplinary field that applies engineering principles to biology, enabling the design, redesign, and construction of new biological parts, devices, and systems or the modification of natural organisms for useful purposes. It combines biology, engineering, genetics, and chemistry to create functional biological systems, such as synthetic bacteria or specialized metabolic pathways.

Article Overview

The paper is a review article that surveys how synthetic biology is being used to design advanced biosensors for healthcare and environmental monitoring. It focuses on:

• Genetic circuits and programmable cells
• CRISPR-based diagnostics
• Cell-free systems
• Wearable/semi-implantable and paper-based devices
• Multiplex detection
• Intelligent, Al-enabled biosensors
• Clinical translation challenges

Abstract

The abstract explains that synthetic biology enables modular, programmable biosensors built from gene circuits, RNA regulators, CRISPR systems, and logic gates. These systems can:

• Detect pathogens, cancer markers, metabolic disorders, and pollutants
• Work in whole-cell or cell-free formats
• Be embedded into wearable or paper-based devices
• Store memory of exposures
• Trigger therapeutic responses

Remaining challenges include stability, biosafety, and scale-up, while future directions include Al integration and hybrid materials.

1. Introduction - Synthetic Biology and Biosensors

1.1 Synthetic Biology as a Game Changer

This section defines synthetic biology as an engineering-driven approach to redesign biological systems using:

• Modular DNA parts
• Gene circuits
• RNA switches
• Logic gates

It explains how these components convert molecular detection into outputs like fluorescence, color changes, or electrical signals, and how cell-free platforms avoid biosafety risks while enabling field deployment.

1.2 Evolution of Biosensing Technologies

Traditional biosensors relied on enzymes and antibodies but lacked flexibility.
Synthetic biology introduced:

• Genetic logic gates
• Oscillators
• Feedback loops
• Multi-layer networks
• Engineered microbes and mammalian cells

It also highlights the shift toward cell-free systems for point-of-care testing and low-resource environments.

1.3 Need for Next-Generation Biosensors
The authors argue that modern healthcare and environmental needs require sensors that are:

• Faster
• More sensitive
• Reprogrammable
• Scalable
• Capable of multiplexing
• Therapeutic-responsive

They justify focusing the review on healthcare and environmental sectors to extract design principles transferable to other fields.

2. Innovations in Synthetic Biology-Driven Biosensing

2.1 Genetic Circuit Engineering

This major section describes how synthetic gene networks are designed to perform sensing and computation.

2.1.1 Fundamental Circuit Types
Digital Circuits
Include:

• Toggle switches
• RNA riboregulators
• Toehold switches
• CRISPR/dCas9 transcriptional control
• Recombinase logic gates
• Boolean logic (AND, OR, NAND, XOR)

These enable decision-making inside cells and multiplexed detection.
Analog Circuits
Produce graded outputs instead of ON/OFF responses.

Examples include:

• Feedback loops controlling transcription factors
• Arsenic sensors with amplification modules
• Hybrid analog-digital converters

Limitations Discussed

• Off-target CRISPR effects
• Recombinase instability
• Narrow dynamic range of RNA switches

2.1.2 Genetic Memory Systems
Explains DNA-based memory tools such as:

• Recombinase recorders
• SCRIBE
• CRISPR self-targeting systems
These allow cells to store exposure histories or track disease states over time.

2.2 Biosynthetic Detection Pathways
Describes circuits that both sense and treat disease.

2.2.1 Mammalian Metabolic Regulators
Examples include:

• Uric-acid-responsive gout therapy circuits
• Light-activated glucose control systems

2.2.2 Multi-Disease Therapeutic Circuits
One synthetic cascade simultaneously addressed:

• Hyperglycemia
• Obesity
• Hypertension
by sensing a drug and releasing multiple therapeutic proteins.

2.2.3 Microbial Platforms
Covers:

• Nitric-oxide detecting gut bacteria
• Arsenic-sensing microbes
• Transporter gene deletions to improve sensitivity

2.2.4 Design Principles
Key principles extracted:

1. Modularity
2. Signal amplification
3. Orthogonality
4. Tunability
Challenges include immune recognition and limited detection ranges.

2.3 Synthetic Organisms and Engineered Proteins
This section explains how:

• Engineered bacteria, yeast, and plants act as sensors
• Proteins are optimized via directed evolution
• Luciferase and transcription-factor hybrids increase sensitivity
• Plants can visibly report pesticide exposure
It also notes biosafety and long-term stability as unresolved issues.

3. Features of Next-Generation Biosensors

3.1 Enhanced Sensitivity and Specificity
Strategies discussed:

• Logic-gated sensing
• Positive-feedback amplification
• Orthogonal receptors
• CRISPR diagnostics
Applications include antibiotic detection, metabolic monitoring, and early infection screening.

3.2 Portability and Real-Time Monitoring
Focuses on:

• Fast transcriptional circuits
• Toehold-switch viral RNA detection
• Cell-free CRISPR platforms
• Wearables
• Feedback-regulated sensors
Examples include glucose monitors and water-quality sensors.

3.3 Multiplexed Detection
Explains systems that detect multiple analytes simultaneously using:

• Orthogonal riboswitches
• Barcoded DNA outputs
• CRISPR Cas13 multiplexing
• Multi-parameter bioprocess monitoring

4.1 Early Disease Detection
Covers:

• Cell-free ribozyme sensors
• COVID-19 riboregulator tests
• Wireless CRISPR electrochemical chips
• Antibiotic-resistance detection
• Pseudomonas diagnostics
It also discusses clinical obstacles:
• Tumor heterogeneity
• Immune reactions
• Complex biological fluids
• Delivery barriers

4.2 Personalized Medicine & Monitoring
Describes:

• Wearable cell-free sensors
• Paper-based blood diagnostics
• Antibiotic-monitoring platforms
• Semi-implantable CRISPR needles for circulating DNA tracking
Overall Message of the Paper
Across all sections, the authors argue that synthetic biology is transforming biosensors into:
• Programmable
• Intelligent
• Multiplexed
• Wearable
• Therapeutic-responsive
but emphasize that biosafety, regulatory approval, robustness, and real-world deployment remain major hurdles.



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February 15, 2026, 12:14:00 PM
 #22


This paper introduces a new routing protocol called DPOR (Data Priority-based Opportunistic Routing) for intra-body nanonetworks - tiny nanoscale devices communicating inside the human body.
The main goal is to improve how medical data moves through these networks by:
• Prioritizing urgent health data (like emergency signals)
• Reducing delay for critical information
° Saving energy of nano-devices
• Preventing overheating that could harm tissues
In short:

The paper designs a smarter way for tiny medical sensors inside the body to send data "safely," efficiently, and according to urgency.

Provided below is a section-by-section overview of the paper:

"DPOR: A data priority- based opportunity routing protocol for intra-body nanonetworks"

https://www.sciencedirect.com/science/article/abs/pii/S1878778925000249

Introduction - Why this research matters
The authors explain that:
• Nanotechnology now allows tiny devices (1-100 nm) to be placed inside the body.
• These devices could monitor health, detect diseases, or deliver drugs.
• Communication between these devices often uses terahertz (THz) electromagnetic signals.
The problem:
• Human tissue and blood interfere with signals.
• Nodes have very limited energy and memory.
• Traditional routing methods don't work well at this scale.
• Some health data is more urgent than other data.
Example:
• Emergency heart rhythm data should arrive faster than normal monitoring data.
Core idea introduced:
Create a routing system that:
1. Understands data urgency
2. Chooses relay nodes intelligently
3. Prevents overheating through energy control
Sec. 2. Related Work - What others have done
This section reviews prior research.
Previous studies focused on:
• Terahertz communication inside tissues
• Energy-efficient routing
• Opportunistic routing
• Thermal-aware protocols (avoiding heat damage)
Limitations in older methods:
• Many ignore data priority.
• Some reduce energy but increase delay.
• Others manage heat but hurt performance.
Gap identified:
No existing protocol combines:
• Data priority
• Energy management
• Temperature control
DPOR aims to combine all three.
3. Intra-Body Nanonetwork Model
This section explains the simulated human-body network.
3.1 3D Pipeline Model
The body's blood vessel is modeled as a 3D pipe.
Inside it:
• Nano-nodes float with blood flow.
• A gateway sits on the vessel wall and collects data.
How movement works:
• Nodes move passively with blood.
• They send data hop-by-hop to the gateway.
• A coordinate system tracks node positions since tiny nodes cannot have GPS.
3.2 Time Relative Position Model
Because nodes can't know exact location:
• The gateway sends periodic signals called Index values.
• Nodes update their Index based on when they receive these signals.
Result:
• Higher Index = closer to gateway
• Lower Index = farther away
This creates a direction for routing without real positioning hardware.
3.3 Energy Harvesting
Nano-nodes recharge themselves using:
• Piezoelectric nano-generators
• Energy from movement inside the body
The paper models:
• How much energy is harvested
• How fast charging happens
• How energy changes over time
This helps prolong network lifetime.
4. DPOR Protocol Design
This is the core of the paper.
4.1 Data Prioritization
Data is divided into three levels:
Priority                  Type                          Example
---------------------------------------
High (p=1)      Emergency      Cardiac arrest signals
Medium (p=2)   Warning             Abnormal vitals
Low (p=3)           Normal             Routine monitoring
---------------------------------------
Key idea:
Different data types get different routing treatment.
High priority → fastest path
Low priority → energy-saving path
4.2 Relay Node Selection
When sending data:
1. A node finds nearby neighbors.
2. It checks:
• Remaining energy
• Distance to gateway (Index)
• Node ID (tie-breaker)
3. It calculates a score to choose the best relay.
Smart behavior:
• Emergency data favors shorter routes → lower delay.
• Normal data favors high-energy nodes → longer network life.
A backoff system prevents collisions between nodes.
4.3 Thermal-Aware Model
Communication creates heat.
Too much heat could damage tissue.
The protocol adds:
• Sleep-wake cycles
• Temperature thresholds
• Energy limits
If a node becomes too hot:
• It sleeps
• Cools down
• Rejoins later
Relay selection also considers node temperature.
This balances safety with performance.
5. Simulation & Performance Results
The protocol was tested using the NS-3 simulator with virtual blood vessels and moving nano-nodes.
5.1 Transmission Delay
Findings:
• DPOR gives lower delay for high-priority data.
• Emergency packets reach the gateway fastest.
• Dynamic Index values help minimize hops.
5.2 Packet Success Rate
DPOR improves reliability because:
• Multiple relay candidates exist.
° ACK and backoff mechanisms reduce failures.
Result: higher successful delivery than older protocols.
5.3 Energy Consumption
Compared to flooding methods:
• DPOR uses less energy.
• Avoids unnecessary transmissions.
• Balances workload among nodes.
5.4 Throughput
Throughput improves because:
• More efficient path selection.
• Reduced packet collisions.
• Priority-aware routing.
5.5 Temperature Behavior
Temperature stabilizes after about 3.5 seconds because:
• Sleep-wake control reduces overheating.
• Energy harvesting balances activity.
This supports biological safety.
5.6 Trade-Offs
The paper honestly notes:
Sometimes DPOR doesn't have the lowest delay.
But it balances:
• Delay
• Energy use
• Thermal safety
This balance is critical for real medical environments.
6. Conclusion - Main Takeaways
The paper concludes that DPOR:
• Prioritizes urgent medical data
• Improves packet success rates
• Reduces energy waste
• Prevents overheating
• Extends nanonetwork lifetime
The authors argue it could support future:
• Smart healthcare
• Body area networks
• Continuous internal monitoring systems
Simple "Big Picture" Summary (Layman's terms)
Imagine tiny medical sensors floating in your blood.
This paper teaches them how to:
• Decide which health information is most important
• Pass messages intelligently like a relay team
• Avoid running out of battery
• Avoid getting too hot and harming tissue
The result is a smarter, "safer" system for in-body monitoring technologies.


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