michaelmclees, it's usually in the manufacture's specification.
On some motherboards pcie x16 and x1 share bandwidth and once you plug something into pcie x16, x1 gets disconnected, but in your case I'd bet the problem is elsewhere. If you you plug only one card in x1 and it doesn't get detected it is probably because you need to short A1 and B17 Pins to trick "Presence Detection". Read about it here
http://blog.zorinaq.com/?e=42