According to NiceHash, using a GEN 3 PCI-E lane can make a 1-2% difference in hash rate from the higher bandwidth, especially for some data transfer intensive algorithms.
We also checked with GPU-Z and all cards were successfully detected and linked with PCI-e v3.0, which is another bonus compared to other motherboards that usually work with 6+ cards only under PCI-e v1.1. The higher bandwidth speed may not look like an important factor, but the slower link can take 1-2% off of card's performance, especially in algorithms that are sending a lot of data from/to CPU/GPU.
https://www.nicehash.com/?p=news&id=155Wouldn't hurt to also do a clean install of the drivers after a DDU. If you move cards around, there are registry entries left in Device Manager that can cause problems.