Yes, but it's probably not that simple (unless you know you only have two funded addresses).
Bitcoin keeps a pool of 100 unused addresses by default. Periodically on its own, and whenever you tell it to, it will "generate" a new address - pull the next one from the pool - and add a new one to the pool to replace it. This is when your backed up wallet becomes desynchronized. As long as you have not used up all 100 pooled addresses since your backup, it should contain the full balance from your regular wallet.
Any coins you spend from the backup will also be spent in your main wallet.